Enhanced amplification of GC-rich DNA with two organic reagents.
نویسندگان
چکیده
Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.
منابع مشابه
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عنوان ژورنال:
- BioTechniques
دوره 47 3 شماره
صفحات -
تاریخ انتشار 2009